By Alain Filloux, Juan-Luis Ramos
In Pseudomonas aeruginosa, professional researchers within the box element a number of the tools that are now regular to review this interesting microorganism. Chapters comprise microbiological tips on how to high-throughput molecular ideas which have been built over the past decade. Written within the hugely profitable Methods in Molecular Biology sequence layout, chapters comprise introductions to their respective themes, lists of the required fabrics and reagents, step by step, comfortably reproducible laboratory protocols and key tips about troubleshooting and keeping off identified pitfalls.
Authoritative and useful, Pseudomonas aeruginosa aids within the carrying on with learn of recent and leading edge findings.
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Additional resources for Pseudomonas Methods and Protocols
FEMS Microbiol Lett 332(2): 162–169 12. Gould JC, McLeod JW (1960) A study of the use of agglutination sera and phage lysis in the classification of strains of Pseudomonas aeruginosa. J Pathol Bacteriol 79:295–311 13. Sillankorva S, Neubauer P, Azeredo J (2010) Phage control of dual species biofilms of Pseudomonas fluorescens and Staphylococcus lentus. Biofouling 26(5):567–575 14. Rhoads DD et al (2009) Bacteriophage therapy of venous leg ulcers in humans: results of a phase I safety trial. J Wound Care 18:237–238 15.
Summers WC (2012) The strange history of phage therapy. Bacteriophage 2(2):130–133 3. Kellenberg G, Kellenberg E (1957) Electron microscopical studies of phage multiplication. III. Observation os single cell bursts. Virology 3:275–285 4. Holloway BW, Egan JB, Monk M (1960) Lysogeny in Pseudomonas aeruginosa. Aust J Exp Biol Med Sci 38: 321–330 5. Ceyssens PJ, Lavigne R (2010) Bacteriophages of Pseudomonas. Future Microbiol 5(7): 1041–1055 6. Pires D et al (2011) Use of newly isolated phages for control of Pseudomonas aeruginosa 32 7.
Carefully place the plate at 37 °C without shaking during 2 h. 4. At the same time, incubate the recipient preculture at 42 °C without shaking during 2 h (see Notes 5 and 6). 3 Conjugation 5. After 2 h, spot 30 μl of the recipient in the fourth compartment (containing the donor and the helper) to initiate the conjugation (Fig. 1b). Place the LB plate at 37 °C without shaking during 4 h (see Note 7). Spot also 30 μl of the recipient preculture in the third compartment (control). 4 Selection 6. Prepare 4 sterile eppendorfs with 400 μl of sterile liquid LB medium.
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