By T.S. Work and E. Work (Eds.)

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Additional resources for Techniques of sample preraration for Liquid Scintillation Counting+Isoelectric Focusing

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Neary and Budd (1970) suggested that the light from a single photon emission will take several different light paths in a coloured sample, resulting in a broadening as observed. However, although Ten Haaf (1972) has attempted to interpret the data in terms of a mathematical model, good experimental support for the theory is still not available. Certain highly coloured organic compounds are best combusted in oxygen, ensuring that complete degradation of the material occurs (Yamazaki et al. 1966).

Manufacturers have been quick to seize on these variations, giving them a multitude of different names compounded in part from the terms ‘phosphor’, ‘scintillant’ and ‘fluor’. A disturbing feature has been the introduction of secret recipes of scintillant mixtures, thus adding an avoidable variable into the liquid scintillation counting technique, which is thereby not exploitable by the research worker should an unusual result occur. An example would be that a secret recipe containing 2-phenyl-5(4‘biphenyl)oxadiazole (PBD) would give erroneous results with certain solubilizers (Dunn 1971) without the worker being able to predict such an event.

2. Heterogenous counting systems A heterogenous system from the point of view of liquid scintillation counting may be defined as a two phase system in which most of the scintillant is dissolved in one phase and most of the beta-emitting sample is incorporated into the second phase. Such a two phase system can be either so1id:liquid or 1iquid:liquid. In the case of the solid :liquid system, the scintillant can be incorporated into either phase; the sample being incorporated into the opposite phase.

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